体内诱导法建立的Tca8113细胞耐卡铂细胞株的耐药性表达研究

目的探讨通过体内诱导法得到的耐药细胞株的耐药性表达情况。方法以人舌癌Tca8113细胞系为研究对象,实验组采用荷瘤耐药动物的肿瘤细胞培养获得,对照组为体外连续培养诱导其产生耐药性。用四唑蓝法检测细胞耐药指数,标记生物素链亲和素法免疫细胞化学检测耐药相关蛋白P糖蛋白、谷胱甘肽S转移酶π、多药耐药蛋白和拓扑异构酶Ⅱ的表达,逆转录PCR检测多药耐药基因、多药耐药蛋白、拓扑异构酶Ⅱ和谷胱甘肽S转移酶π的表达。结果免疫细胞化学和逆转录PCR显示2组细胞多药耐药蛋白、谷胱甘肽S转移酶π、拓扑异构酶Ⅱ等表达均升高,但体内诱导较体外诱导可以获得更高、更稳定的耐药性。诱导后的Tca8113细胞对氨甲喋呤、卡铂、平阳霉素、长春新碱耐药性明显升高,而对5-氟尿嘧啶耐药性增加不明显。结论体内诱导得到的细胞株耐药性明显高于体外诱导株,肿瘤细胞的耐药现象受多种基因的调节。体内诱导Tca8113卡铂耐药细胞株Tca8113/CBP-vo可以作为肿瘤耐药研究的平台。     

Potential function of TGF-β isoforms in maturation-stage ameloblasts

Objectives

To investigate potential functions of transforming growth factor-beta (TGF-β) isoforms in maturation-stage ameloblasts during amelogenesis.

田基黄对人舌癌细胞株TSCCa细胞毒作用的研究

本实验采用体外细胞毒试验（MTT法）和电镜观察，研究了田基黄对人舌癌细胞株TSCCa的细胞毒作用。结果表明：田基黄对人舌癌细胞株TSCCa的生长有明显的抑制作用，其抑制率随浓度的增加而提高。电镜下见田基黄对癌细胞内线粒体和粗面内质网有损伤作用。结果提示中药田基黄对口腔鳞癌有较好的治疗作用。     

Shear bond strength of composite resin cements to lithium disilicate ceramics

The aim of this study was to evaluate the shear bond strength of different commercial composite resin cement systems to lithium disilicate all-ceramic substrate. Five adhesive resin cement systems Panavia 21 and Panavia F (Kuraray), Variolink 2 (Ivoclar-Vivadent), RelyX Unicem Applicap and RelyX ARC (3M ESPE) were used on all-ceramic (IPS Empress 2; Ivoclar-Vivadent) substrate. Shear bond strength of adhesive resin cement to substrate was tested after thermocycling, or without thermocycling (n = 10). Substrate surfaces of the specimen after loading were SEM microscopically examined. The highest bond strengths in water stored conditions were obtained with RelyX ARC (28.7 +/-3.9 MPa), while in thermocycled conditions the highest bonding values were obtained with Variolink 2 (23.2 +/- 7.5 MPa). The lowest values in both water stored (5.8 +/- 4.0 MPa) and thermocycled (2.4 +/- 2.9 MPa) conditions were obtained with Panavia 21. Shear bond strengths appeared to be affected significantly by thermocycling (anova, P < 0.05). It was concluded that there were significant differences between the bond strengths of adhesive resin cements to lithium disilicate substrate.     

三种陶瓷酸蚀剂对Cerinate瓷和树脂粘结强度的影响

目的 :比较三种陶瓷酸蚀剂及不同酸蚀时间对Cerinate瓷与树脂粘结强度的影响。方法 :将Cerinate瓷试件分为 4组 ,一组不使用酸蚀剂 ,其余三组分别使用自制A型 (含 2 .5 %氢氟酸 )、B型 (含 6%氢氟酸 )及C型(含 10 %氢氟酸 )陶瓷酸蚀剂 ,分别酸蚀 3 0s～ 180s ,每 3 0s为一间隔 ,与树脂粘结后测试剪切强度。结果 :C配方陶瓷酸蚀剂在 3 0s时粘结强度最大 ,达 2 7.64MPa ,相应瓷表面形态最适于粘结。结论 :C配方 (含 10 %氢氟酸 )陶瓷酸蚀剂具有较好的临床应用价值。     

瓷表面不同处理方法及粘结剂对全瓷剪切强度的影响

目的:观察喷砂、酸蚀及硅烷偶联剂等表面处理方法对热压铸陶瓷,玻璃渗透陶瓷和氧化锆陶瓷与不同种粘结剂的粘结强度的影响。方法:样本分为热压铸陶瓷组,渗透陶瓷组和氧化锆陶瓷组。分别应用不同表面处理方法和粘结剂,测其剪切强度。结果:方差分析表明,热压铸陶瓷组中酸蚀涂硅烷树脂粘结组粘结强度最高,渗透陶瓷组中喷砂玻璃离子粘结组粘结强度最高,氧化锆陶瓷组中喷砂玻璃离子粘结组粘结强度最高。结论:应用常规树脂粘结剂粘固热压铸陶瓷修复体取得较好的粘固效果,采用酸蚀和硅烷偶联剂的表面处理,可取得最大粘结强度。     

人涎腺多形性腺瘤细胞体外培养及其生物学特性

目的 建立人涎腺多形性腺瘤细胞系。方法 取自腮腺多形性腺瘤患者的原发肿瘤进行体外培养。对培养的细胞进行活细胞观察和组织学染色。观察细胞生长状况，绘制生长曲线。采用免疫细胞化学检测细胞内特异性抗原标记物对细胞进行鏊定，并对肿瘤的致瘤性进行裸鼠异种移植实验。结果 多形性腺瘤体外培养细胞呈多边形或梭形，有的细胞围成腺腔结构，胞浆内富含粘液，形成空泡。有的成片排列，细胞外有粘液样物质。细胞的生长曲线较平缓，无明显快速生长期。肿瘤性腺上皮细胞角蛋白阳性，肿瘤性肌上皮细胞肌动蛋白阳性。结论 建立的涎腺多形性腺瘤有限细胞系(SPA-02)，保留了原代细胞的特点，并与原发肿瘤的组织学特征相似。     

Shear bond strengths of saliva contaminated 'one-bottle' adhesives

The aim of this study was to investigate the effect of saliva contamination on the bond strengths of three one-bottle bonding systems. The dentin of 90 recently extracted, non-carious human molar teeth was exposed and ground wet on 500 grit silicon carbide (SIC) paper to establish a bonding surface in superficial dentin. Specimens were randomly assigned to nine groups of 10 teeth each. Three testing conditions are: (i) contamination with fresh saliva (pH 5.5) after acid etching, (ii) contamination with fresh saliva after bonding application, and (iii) no contamination. Three adhesive systems are: syntac single component, prime & bond NT, and gluma one bond. Cylinders of composite were applied via PTFE (polytetrafluroethylene) split mould and light cured to the dentin surfaces. All specimens were thermocycled (5000 cycles) between baths of 5 and 55 degrees C. Shear bond strengths were measured using an Instron Universal testing machine at a crosshead speed of 1 mm min-1. Two-way ANOVA was used to analyse the data. The results of this study indicated that the saliva contamination of dentin has no adverse effect on the bonding efficiency of one-bottle adhesive systems (P > 0.05). No statistically significant differences were found between the shear bond strengths of tested adhesives.     

PTTG和bFGF在舌癌细胞株Tca8113中表达相关性研究

目的：探讨垂体肿瘤转化基因（Pituitary Tumor Transforming Gene,PTrG）蛋白在舌癌细胞株Tca8113中的表达及其与碱性成纤维细胞生长因子（basic Fibroblast Growth Factor,bFGF）间的关系.方法：应用免疫细胞化学方法,检测加入bFGF抗体后孵育的癌细胞中PTTG蛋白表达的变化.结果：Tca8113中PTTG蛋白呈阳性表达,并受bFGF影响,加入bFGF抗体的培养液中的癌细胞PTTG蛋白荧光明显减弱,随着时间延长和bFGF抗体滴度增加,减弱趋势明显.结论：舌癌细胞株中PTTG蛋白的表达受bFGF制约,对二者的联合治疗可能是一种治疗舌癌的有效方法.